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How the Globe tested fish DNA

To conduct DNA testing of fish, the Boston Globe hired the Biodiversity Institute at the University of Guelph, which houses the International Barcode of Life. This 26-nation consortium uses a novel method of genetic analysis to quickly and inexpensively identify species. The method compares a snippet of a specimen’s DNA against an existing DNA library to identify the species. Scientists use a fragment of a gene found in a cell’s mitochondria that is short enough to be quickly sequenced, but long enough to vary significantly from one species to another. The technique is called DNA barcoding because, just as a supermarket scanner reads a barcode to distinguish a can of beans from a carton of milk, the DNA snippet separates one species from another.

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