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NEW YORK — In an effort to counter US sanctions, Iran will pull further away from a landmark nuclear accord signed four years ago, President Hassan Rouhani said Tuesday, announcing that the country would inject uranium gas into more than 1,000 centrifuges at a highly secure site that Iran initially hid from inspectors.

Injecting the gas into the centrifuges at the Fordow nuclear facility is a step toward uranium enrichment, which in turn moves Iran closer to being able to build a nuclear weapon, though it has denied harboring such ambitions.

President Trump withdrew the United States from the accord last year and imposed sanctions in an effort to put pressure on the government in Tehran. In response, Iran has taken several calibrated steps this year to exceed the limits the agreement had put on its nuclear program, while calling on Trump to reverse himself.

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Iran will begin injecting uranium gas into the 1,044 centrifuges at the Fordow nuclear facility, Rouhani said; the 2015 agreement had restricted the centrifuges to uses other than nuclear enrichment.

The announcement came a day after Iran said it had doubled the number of more advanced centrifuges operating at its main nuclear plant, at Natanz, and planned to install more efficient centrifuges.

The Iranian president portrayed the announcement Tuesday — as well as three previous steps it had taken beyond the deal — as reversible, but Iran has made it clear that it will only step back if the European signatories to the deal find a way to relieve the economic pressure caused by US sanctions.

“Resistance lays the ground for negotiation, and negotiation takes advantage of resistance,” Rouhani said, according to the state-run IRNA news agency.

Iran has recently gone beyond the limitations of the nuclear agreement by increasing its nuclear stockpile, enriching uranium at higher levels, and enlarging the number of centrifuges in use. Fordow was built deep under a mountain outside the holy city of Qom, and Iran did not acknowledge its existence openly until 2009. The nuclear agreement allowed the centrifuges at the facility to remain in use on the condition that gas was not injected into them.

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Iran might be able to produce enough fuel for a nuclear bomb in under a year, according to some analysts, and it was not immediately clear how the announcement Tuesday would change the equation.

Sanam Vakil, a research fellow at Chatham House’s Middle East and North Africa program, said that Iranian authorities had remained “relatively calibrated” with the announcement Tuesday.

“Iran is pushing the red lines, as part of its own maximum pressure campaign to reverse Europe’s position. But Iran could have done more and they’re not,” she said. “This is still one those steps that Iran can reverse.”

Under the 2015 deal, Iran has been allowed to enrich uranium with 5,000 first-generation centrifuges, known as IR-1 at Natanz.

The additional 1,044 centrifuges at Fordow are also IR-1, which are seen by experts as antiquated. The more advanced centrifuges, known as IR-6, refine uranium 10 times faster, according to Iranian authorities, and Iran said Monday that it had started injecting gas into them at the Natanz plant.

The US sanctions, which Rouhani described as “wrong, cruel, and illegal,” delivered another blow to an already fragile Iranian economy, and the Iranian government has responded with what is effectively a two-track strategy.

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The agreement was signed in 2015 by Iran and the United States, along with China, France, Russia, Britain, Germany, and the European Union. Iran agreed to reduce the size of its nuclear program in exchange for relief from sanctions.

Trump, in withdrawing from the pact, reimposed old sanctions and later added new ones. Iran has argued that it cannot abide by the deal as long as the sanctions are crippling its economy, while at the same time saying it would adhere to the agreement if the European partners to the nuclear agreement could lessen their effect.

The European signatories, which still support the deal, have sought to find ways to address the Iranian concerns but have been unable to come up with alternatives that satisfy Tehran without running afoul of the US sanctions.

Maja Kocijancic, a spokeswoman for the European Commission, the European Union’s executive arm, said the EU was “concerned” about the announcement, urging Iran to reverse earlier breaches of the nuclear deal and refrain from any further moves that would undermine it.

Dmitry Peskov, the Kremlin spokesman, said that Russia would like to see the nuclear deal remain in force, but he also expressed sympathy for Iran, citing the “unprecedented and illegitimate sanctions” against it.