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BRUSSELS — Britain, France, and Germany on Tuesday formally accused Iran of breaking the 2015 agreement that limited its nuclear program, taking the first step toward reimposing United Nations sanctions.

The European countries started the clock running on what could be some 60 days of negotiations with Iran about coming back into full compliance with the nuclear deal. Under the agreement, if they cannot resolve their dispute, that could revive UN sanctions on Iran that had been suspended under the deal, including an arms embargo.

The move, which had been expected for more than a week, was delayed when the United States killed a top Iranian commander, General Qassem Soleimani, with repercussions that are still playing out in Iran and across the region.

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President Trump withdrew in 2018 from the deal, which was negotiated under President Barack Obama, and he has imposed several rounds of US sanctions on Iran. In response, Tehran has repeatedly moved beyond the limits that the agreement had placed on its uranium enrichment, raising fears that it could be close to building an atomic bomb.

The Europeans want to save the deal and persuade both Washington and Tehran to begin a new set of negotiations about missile development and Iran’s regional activities, a senior European official said.

But the three European countries, all signatories to the deal, known as the Joint Comprehensive Plan of Action, or JCPOA, clearly felt they had to respond to Iran’s movement away from compliance.

On Tuesday, Iran’s Foreign Ministry warned of a “serious and strong response” to the European decision. At the same time, its spokesman Abbas Mousavi said that Iran was “fully ready to answer any goodwill and constructive effort” that would preserve the nuclear deal, according to the official IRNA news agency.

In a joint statement, the foreign ministers of Britain, France, and Germany said they had warned Iran on Dec. 6 that “unless it reversed course, we would have no choice but to take action,” but Iran “has chosen to further reduce compliance.”

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On Tuesday, they set in motion the nuclear deal’s dispute resolution process, they said, “in good faith, with the overarching objective of preserving the JCPOA and in the sincere hope of finding a way forward through constructive diplomatic dialogue.”

The three countries reiterated that they opposed Trump’s withdrawal from the deal and were not joining his campaign of “maximum pressure” to cripple Iran economically. “Our hope is to bring Iran back into full compliance,” they said.

There was no immediate reaction from Tehran.

The 2015 agreement restricted how many centrifuges Iran could use to enrich uranium — increasing the percentage of U-235, the rare isotope crucial to its use in nuclear fission — how highly it could enrich the metal, and how much uranium it could stockpile.

It has taken a series of steps beyond those limits, trying in vain to pressure the Europeans to make good on a commitment to ease the economic pain of US sanctions.

Earlier this month, in response to the Soleimani killing, Iran said it would no longer abide by any restrictions in its uranium enrichment, but without specifying what it would actually do, which was taken by the Europeans as a useful ambiguity.

Trump’s harsh sanctions include a prohibition on banking transactions with Iran — a very difficult prohibition to work around, given the global reach of US banks.

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The Europeans have struggled to get a barter system working to circumvent the use of the dollar and US banking systems, adding to Iranian frustration as the US sanctions take a toll on the country.

The Europeans, especially Prime Minister Boris Johnson of Britain, want to give a nod toward Trump while also trying to bring urgency to diplomatic efforts to get new talks underway.

In a BBC interview Tuesday morning, Johnson spoke flatteringly of Trump and said he wanted to avert further military confrontation between Iran and the United States. “Let’s dial this thing down,” he said.

“President Trump is a great deal-maker, by his own account,” Johnson said. “Let’s work together to replace the JCPOA and get the Trump deal instead,” he added, though it was not clear what such a deal would look like.

In a separate statement, Foreign Minister Heiko Maas of Germany said, “We could no longer leave unanswered the increasing Iranian violations of the nuclear agreement unanswered.”

“Our objective is clear: We want to preserve the agreement and reach a diplomatic solution within the agreement,” he said. “We will tackle this together with all partners in the agreement. We call on Iran to participate constructively in the negotiation process that is now beginning.”

But there was some skepticism that the time was right for talks.

Robert Malley, who heads the International Crisis Group and helped negotiate the nuclear deal, said on Tuesday that Trump and his aides would feel now that Iran was weakened by the sanctions and the protests in the streets after the shooting down of a Ukrainian airliner. The Americans would be unlikely to want to give Iran any concessions to start the talks, he said, let alone the lifting of US sanctions that Iran insists must be a precondition.

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At the same time, Malley said, senior Iranian officials would not want to be seen talking with Trump administration officials responsible for the killing of Soleimani, a popular figure in Iran who was considered its second most important leader.

“The Europeans are trying not to offend Trump too much but also trying to keep the JCPOA alive,” he said. “But the US now thinks it’s winning,” he said, “so the Europeans are trying now to avoid it all going off the rails.”