DONETSK, Ukraine — Men in the uniforms of Ukraine’s now-defunct riot police on Saturday occupied police headquarters in Donetsk, the eastern city that is one of the flashpoints of a wave of pro-Russia protests, hours after armed men seized local police headquarters and a local branch of the Security Service in a nearby city.
Interior Minister Arsen Avakov described the unrest as ‘‘Russian aggression’’ and said Ukraine sent special forces to the area to deal with the situation.
The unrest in Donetsk and the city of Slovyansk, about 55 miles to the north, were the latest shows of spiraling anger in eastern Ukraine, which has a large Russian-speaking population and was also the support base for Viktor Yanukovych, the Ukrainian president who was ousted in February after months of protests in the capital, Kiev.
Ethnic Russians in Ukraine’s east widely fear that the authorities who took over after Yanukovych’s fall will suppress them.
In Slovyansk, the mayor said the men who seized the police station were demanding a referendum on autonomy and possible annexation by Russia. Protesters in other eastern cities have made similar demands after a referendum in Crimea last month in which voters opted to split off from Ukraine, leading to annexation by Russia.
Witnesses said the men who entered the police building in Donetsk were wearing the uniforms of the Berkut, the feared riot police squad that was disbanded in February after Yanukovych’s ouster.
Berkut officers’ violent dispersal of a demonstration in Kiev in November set off vast protests in the capital that culminated in bloodshed in February when more than 100 people died in sniper fire; the acting government says the snipers were police.
It was not immediately clear if the men who occupied the Donetsk police building had made any demands, but the Donetsk police chief said on national television that he was forced to offer his resignation. Interfax Ukraine reported that pro-Russian protesters had invited the former police chief to resume his duties.
In Slovyansk, about 20 men in balaclavas and armed with automatic rifles and pistols were guarding the entrance to the police station in the city of about 120,000 people, and another 20 were believed to be inside. They wore St. George’s ribbons, which have become a symbol of pro-Russian protesters in eastern Ukraine. The ribbons were originally associated with the Soviet Union’s victory in World War II.
A masked guard in Slovyansk, who gave his name only as Sergei, said they have ‘‘only one demand: a referendum and joining Russia.’’
The man said they seized the building because they wanted to protect it from radical nationalists from western Ukraine and ‘‘the junta who seized power in Kiev.’’
‘‘We don’t want to be slaves of America and the West,’’ he said, speaking at the seized police station. ‘‘We want to live with Russia.’’
Avakov said in a Facebook post Saturday evening that unknown men opened fire on a police station in Kramatorsk, a town near Slovyansk, and police were engaged in a gunfight with them. In Krasnyi Lyman, another town in the area, men armed with Russian-made automatic rifles attacked a police station, he said.
The Interior Ministry said the attackers in Slovyansk used tear gas and stun grenades when they stormed the building, injuring three police officers.
The attackers’ goal was to seize arms from the police station, authorities said, adding that there were about 40 automatic rifles and 400 pistols as well as ammunition inside.
The Interior Ministry reported later Saturday that men from the same group have seized the building of the local Security Service.
The Kiev authorities and the United States have accused Russia of fomenting the unrest in the east and seeking to use it as a pretext for sending in troops. Russia has reportedly massed forces in areas near the Ukrainian border.
But Slovyansk Mayor Nelya Shtepa said she held talks with the protesters and said they were local residents, not Russians.
‘‘They told me: ‘We don’t have anything against you,’ ” she said, adding that the men said they ‘‘want to be heard, want a dialogue with authorities in Kiev.’’
Russia’s Foreign Ministry on Friday warned the Ukrainian government against using force against protesters, saying that such action would derail the talks on settling the crisis between the United States, the European Union, Russia, and Ukraine set for next week, as well as any other diplomatic efforts.